(2014). Prof. Dr. Michael Hust Technische Universität Braunschweig Institut für Biochemie, Biotechnologie und Bioinformatik Abteilung Biotechnologie Spielmannstraße 7 38106 Braunschweig Tel. 38106 Braunschweig Kai-Thomas Schneider and Nora Langreder arrived in a good mood and with mouth and nose protection for our conversation via video conference. Prof. Dr. Michael Hust Technische Universität Braunschweig Institut für Biochemie, Biotechnologie und Bioinformatik Abteilung Biotechnologie Spielmannstraße 7 38106 Braunschweig Tel. Lecturer for molecular biotechnology. Persistence of SARS-CoV-2 specific B-and T-cell responses in convalescent COVID-19 patients6-8 months after the infection. (68) Bertz, A., Wöhl-Bruhn, S., Miethe, S., Tiersch, B., Koetz, J., Hust, M., Bunjes, H. and Menzel, H. (2013). Normally, the corresponding approval procedure takes about one and a half years or more. Expression rekombinanter Proteinpharmazeutika. In: Antibody engineering Vol 2, Ed: Kontermann, R. and Dübel, S.. (45) Mollova, S., Retter, I., Hust, M., Dübel, S. and Müller, W. (2010). Sequencedefined antibodies improve the detection of Cadherin 2 (N-Cadherin) during Zebrafish development. C., Jordan, E., El-Ghezal, A., Schirrmann, T., Hust, M. und Jostock, T. (2005). (100) Schieferdecker, A., Oberle, A., Thiele, B., Hofmann, F., Göthel, M, Miethe, S., Hust, M., Braig, F., Voigt, M., Koch-Nolte, F., Haag, F., Alawi, M., Indenbirken, D., Grundhoff, A., Bokemeyer, C., Bacher, U., Kröger, N. and Binder, M. (2016). Isolation and characterisation of a human-like antibody fragment (scFv) that inactivates VEEV in vitro and in vivo. and Rhiel, E. (1999). There, SARS-CoV-2 viruses are used to test whether the antibodies can block these viruses. Die TU Braunschweig (Prof. Michael Hust) ist Teil des COVID19-Konsortiums "ATAC" im Rahmen des EU Horizon2020 Corona-Förderprogramms. Bildnachweis: Michael Hust/TU Braunschweig. (34) Pelat, T., Hust, M., Hale, M., Lefranc, M.-P., Dübel, S. and Thullier, P. (2009). Phone: +49 531 391-5731 BIOSpektrum (Springer-Verlag, Heidelberg). Ausbildung und Beruf. Human antibodies targeting CD30+ lymphomas. When we are ill, our immune system forms antibodies against the pathogens (antigens). Regulatory T cells engineered with a novel insulin-specific chimeric antigen receptor as a candidate immunotherapy for type 1 diabetes. In addition, the team has also created such libraries from the blood of cured corona patients because the probability of finding a suitable antibody is even greater in such cases. (105) Miethe, S., Mazuet, C., Liu, Y., Tierney, R., Rasetti-Escargueil, C., Avril, A., Frenzel, A., Thullier, P., Pelat, T., Urbain, R., Fontayne, A., Sesardic, D., Hust, M. and Popoff, M.-R. (2016). Journal of Microbiological Methods 38, 69-80, (1) Janssen, M., Hust, M., Rhiel, E. and Krumbein, W.E. since 12/2002 Group leader at the Institut für Biochemie und Biotechnologie, Abteilung Biotechnologie, Technische Universität Braunschweig headed by Prof. Dr. Stefan Dübel. Isolation of a human-like antibody fragment (scFv) that neutralizes ricin biological activity. Isolation of a nanomolar scFv inhibiting the endopeptidase activity of botulinum toxin A, by single-round panning of an immune phage-displayed library of macaque origin. Production of single chain Fab (scFab) in Bacillus megaterium. (56) Lüders, S., David, F., Steinwand, M., Jordan, E., Hust, M., Dübel, S. and Franco-Lara, E. (2011). TU Braunschweig is involved in the “PhoenixD” cluster at Leibniz Universität Hannover (LUH), which is researching new optical technologies. Selection Strategies II: Antibody phage display. “Before Corona, each of us did our own research on his or her own project. Discovery of Leptospira spp. 47 of the best inhibiting antibodies were handed over to the HZI for further testing. Jacobsen and Dr. Thomas Reinard. Suppression of p75 Neurotrophin Receptor Surface Expression with Intrabodies Influences Bcl-xL mRNA Expression and Neurite Outgrowth in PC12 Cells. Recombinant VHHA6 cDNA was cloned into the pCMX expression vector (contributed by M. Hust, TU Braunschweig), which encodes an optimized human IgG1 Fc domain. (97) Connor, D.O., Zantow, J., Hust, M., Bier, F.F. Spielmannstraße 7 Norman Hack zusammen mit weiteren Instituten aus Braunschweig, München und Hannover Grundlagenforschung zum Thema „Additive Fertigung von Bauteilen“ betrieben. (138) Wenzel, E.V., Roth, K.D.R., Russo, G., Fühner, V., Helmsing, S., Frenzel, A. and Hust, M. (2020). Diese Probe aufs Exempel, müssen dann Andere machen, denn Husts Labor an der TU fehlt die nötige Sicherheitsstufe, um mit dem hochinfektiösen Virus „SARS-CoV-2“ zu experimentieren. (2013). The two PhD students Nora Langreder and Kai-Thomas Schneider work on the CORAT consortium. Phage display, Ed: Lim, T.S. (89) Droste, P., Frenzel, A., Steinwand, M., Pelat, T., Thullier, P., Hust, M., Lashuel, H. and Dübel, S. (2015). With this information, we can produce them again and again.”, This small tube contains an antibody library with about 10 billion antibody blueprints. In Braunschweig, so Hust, kann dies nur das Helmholtz-Zentrum für Infektionsforschung, einer seiner Partner. Massensensitives Sensor-Fließsystem zur CRP-Diagnostik. (94) Unkauf, T., Miethe, S. Fühner, V., Schirrmann, T., Frenzel, A. and Hust, M. (2016). Perspectives for systematic in vitro antibody generation. (19) Pelat, T., Hust, M., Laffly. (115) Rasetti-Escargueil , C., Avril, A., Miethe, S., Mazuet, C., Derman, Y., Selby, K., Thullier, P., Pelat, T., Urbain, R., Fontayne, A., Korkeala, H., Sesardic, D., Hust, M. and Popoff, M.R. (2016). (122) Moreira, G.M.S.G., Fühner, V. and Hust, M. (2018). (135) Russo, G., Fühner, V., Frenzel, A., Hust, M. and Dübel, S. (2019) Parallelized Microscale Expression of Soluble scFv. Efficient production of soluble recombinant single chain Fv fragments by a Pseudomonas putida strain KT2440 cell factory. and Dübel, S. (2018). Microsensor System for Biochemical and Medical Analysis. Email: s.duebel@tu-braunschweig.de - identification of disease related biomarkers (immunogenic oligopeptides) using phage display, - develoment of an anti-tick vaccine (GO-Bio8 project) (Info Video). Affinity maturation by phage display. The research of the TU scientists in the consortium contributes to the research focus “Infection and Therapeutics” at the TU Braunschweig. Pyruvate dehydrogenase complex - enzyme 2, a new target for. In addition, the team has also created such libraries from the blood of cured corona patients because the probability of finding a suitable antibody is even greater in such cases. Zur anonymisierten Webanalyse nutzt die TU Braunschweig die Software Matomo. Bacterial flagellar capping proteins adopt diverse oligomeric states. A., Schirrmann, T., Dübel, S. and Hust, M. (2013). (71) Jäger, V., Büssow, K., Wagner, A., Weber, S., Hust, M., Frenzel, A. and Schirrmann, T. (2013). (16) Jordan, E., Hust, M., Roth, A., Biedendieck, R., Schirrmann, T., Jahn, D. and Dübel, S. (2007). (2016). Generating recombinant antibodies for research, diagnostics and therapy using phage display. An der TU Braunschweig werden – im Gegensatz zu den bisher meist in Tieren erzeugten Antiseren – rein menschliche Antikörper mithilfe der Antikörperphagendisplay-Technologie entwickelt. Influence of the hydromechanical stress and temperature on growth and antibody fragment production with Bacillus megaterium. www.bbt.tu-bs.de/Biotech, Scientists and industrial partners in the Corona Antibody Team (CORAT consortium) are researching a passive immunisation therapy against the corona virus. PMID: 31953428 PMCID: PMC6969050 DOI: 10.1038/s41598-019-57103-5 Abstract Diphtheria is an infectious disease caused by Corynebacterium diphtheriae. “The next step will be clinical trials, which hopefully can start in autumn,” says Stefan Dübel. and Hust, M.. (121) Zantow, J., Moreira, G.M.S.G., Dübel, S. and Hust, M. (2018. (111) Froude, J.W., Pelat, T., Miethe, S., Zak, S.E., Wec, A.Z., Chandran, K., Brannan, J.M., Bakken, R.R., Hust, M., Thullier, P. and Dye, J.M. B., Hust, M. and Zielonka S. (in press). Department of Biotechnology (52) Schirrmann, T., Meyer, T., Schütte, M., Frenzel, A., and Hust, M. (2011). Journal of Virological Methods 106, 225-233, (2) Hust, M., Krumbein, W.E. Recombinant Antibodies for Diagnostics and Therapy against Pathogens and Toxins generated by Phage Display. Supernatant was harvested after 48 h. A high affinity, human like antibody fragment (scFv) neutralising lethal factor (LF) of Bacillus anthracis by inhibiting the PA-LF complex formation. (80) Marschall, A.L.J., Zhang, C., Frenzel, A., Schirrmann, T., Hust, M., Perez, F. and Dübel, S (2014). Immunotherapy of Cancer: Methods and Protocols, Ed. Picture credits: Michael Hust/TU Braunschweig. Antibody Phage Display: Antibody Selection in Solution Using Biotinylated Antigens. Obtention and engineering of non-human primate (NHP) antibodies for therapeutics. (139) Wenzel, E.V., Bosnak, M., Tierney, R., Schubert, M., Brown, J., Dübel, S., Efstratiou, A., Sesardic, D., Sticking, P. and Hust, M. (2020). So-called antibody gene libraries contribute to this. In cooperation with partners from research institutes, university hospitals and companies, the team is looking for ways and means to shorten these processes and still comply with all necessary safety and quality requirements for drugs. Fructose 1,6-Bisphosphate Aldolase, a Novel Immunogenic Surface Protein on Listeria Species. Email: m.hust@tu-braunschweig.de (2011). (37) Thie, H., Binius, S., Schirrmann, T., Hust, M. and Dübel, S. (2009). Single chain antibodies as tools to study TGF-β regulated SMAD proteins in proximity ligation-based pharmacological screens. Picture credits: Michael Hust/TU Braunschweig. (72) Frenzel, A., Hust, M. and Schirrmann, T. (2013). Braunschweig TU Braunschweig ATAC (Antibody therapy against corona) Prof. Michael Hust TU Braunschweig, Karolinska Institut (Schweden), IRB (Schweiz), Joint Research Center Immunochemical Protocols, 3rd ed., Ed: Burns, R., Methods Molecular Biology 295, 71-95, (5) Hust, M. and Dübel, S. (2004). (64) Meyer, T., Schirrmann, T., Frenzel, A., Miethe, S., Stratmann-Selke, J., Gerlach, G.F., Strutzberg-Minder, K., Dübel, S., and Hust, M. (2012). Sprachen und interkulturelle Kommunikation, Graduiertenschulen und Graduiertenkollegs, Fakultät Architektur, Bauingenieurwesen und Umweltwissenschaften, Fakultät für Elektrotechnik, Informationstechnik, Physik, Fakultät für Geistes- und Erziehungswissenschaften, Institut für Biochemie, Biotechnologie und Bioinformatik, Institut für Chemie und Biologie des Meeres, Institut für Biochemie und Biotechnologie, Abteilung Biotechnologie, Technische Universität Braunschweig, Advances in Experimental Medicine and Biology. Detection and Quantification of ADP-Ribosylated RhoA/B by Monoclonal Antibody. Researchers at the Department of Biotechnology of Technische Universität Braunschweig started a research project to develop novel antibody based therapy for coronavirus infections. (131) Fühner, V., Heine, P.A., Zilkens, K., Meier, D., Roth, K.D.R., Moreira, G.M.S.G., Hust, M. and Russo, G. (2019). (109) Noyan, F., Zimmermann, K., Hardtke-Wolenski, M., Knoefel, A., Schulde, E., Geffers, R., Hust, M., Huehn, J., Galla, M., Morgan, M., Jokuszies, A., Manns, M.P., Jaeckel, E. (2017). Kontakt. On the influence of vector design on antibody phage display. & Schirrmann, T. (2020) Rekombinante, vollständig menschliche Antikörper zur Behandlung akuter COVID-19. Transient plant production of Salmonella Typhimurium diagnostic antibodies. Erfahren Sie mehr über die Kontakte von Wolfgang Niemeier und über Jobs bei ähnlichen Unternehmen. Human antibody gene libraries. Vom Hiwi-Job bis zur Professur: Hier finden Sie unsere Stellenangebote und unser Profil als Arbeitgeberin. In: Handbook of therapeutic antibodies, Ed. Cetuximab (Erbitux). (77) Hust, M., Frenzel, A., Schirrmann, T. and Dübel, S. (2014). Generating recombinant antibodies to the complete human proteome. C., Jordan, E., El-Ghezal, A., Schirrmann, T., Hust, M. und Jostock, T. (2005). Wirkstoff-Kandidat von Forschern der TU Braunschweig geht in klinische Phase. (46) Steinwand, M., Jordan, S. and Hust, M. (2010). A total of 20 µg CMX VHH-Fc plasmid DNA and 60 µL PEI (1 mg/mL) were used to transiently transfect 5 × 10 6 HEK293T cells. Phage Display and selection in microtitre plates. Wir sind auch dankbar für die rasche Anschubfinanzierung aus dem Land Niedersachsen, und die Förderung durch die Deutsche Herzstiftung e.V., Frankfurt a.M. (102) Kuhn, P., Fühner, V., Moreira, G.M.S.G., Frenzel, A., Miethe, S. and Hust, M. (2016). The production of a genus specific recombinant antibody (scFv) using a recombinant Potyvirus protease. Institute of Biochemistry, Biotechnology and Bioinformatics Human Anti-Lipopolysaccharid (LPS) antibodies against Legionella with high species specificity. (96) Kibat, J., Schirrmann, T., Knape, M.J., Helmsing, S., Meier, D., Hust, M., Schröder, C., Bertinetti, D., Pardes, K., Funk, M., Lages, A., Giese, N., Herberg, F. W., Dübel, S. and Hoheisel, J.D. (22) Schirrmann, T., Hust, M. and Dübel, S. (2007). “This is a method of producing antibodies in the test tube without the immunisation of animals,” explains Stefan Dübel, one of the inventors of the method. (101) Zantow, J., Dübel, S. and Hust, M. (2016). (103) Frenzel, A., Schirrmann, T. and Hust, M. (2016). Enrichment of open reading frames presented on bacteriophage M13 using Hyperphage. Generation and characterization of protective antibodies to Marburg virus. (87) Kügler, J., Wilke, S. Meier, D., Tomszak, F., Frenzel, A. , Schirrmann, T., Dübel, S., Garritsen, H., Hock, B., Toleikis, L., Schütte, M. and Hust, M. (2015). (36) Hust, M., Steinwand, M., Al-Halabi, L., Helmsing, S., Schirrmann, T. and Dübel, S. (2009). (113) Kuhn, P., Thiem, S., Steinert, M., Purvis, D., Lugmayr, V., Treutlein, U., Plobner, L. , Leiser, R.-M., Hust, M. and Dübel, S. (2017). The antibodies with the best characteristics are then passed on to the Helmholtz Centre for Infection Research (HZI). Production of Single Chain Fragment Variable (scFv) Antibodies in Escherichia coli Using the LEX Bioreactor. This way, people who are already ill could be cured and even risk patients or medical staff could be protected. Generation of recombinant antibodies for human tissue kallikrein 7 to address skin diseases. Even supposedly minor tasks such as ordering materials or sterilizing work equipment are important for research to function. (15) Soltes, G., Hust, M., Ng, K.K.Y., Bansal, A., Field, J., Stewart, D.I.H., Dübel, S., Cha, S. and Wiersma, E. (2007). Cell-free eukaryotic systems for the production, engineering and modification of scFv antibody fragments. In: Antibody engineering Vol 1, Ed: Kontermann, R. and Dübel, S.. (43) Hust, M. and Mersmann, M. (2010). ORFeome Phage Display. Selection of recombinant antibodies from antibody gene libraries. Development of Germline-Humanized Antibodies Neutralizing Botulinum Neurotoxin A and B. Development of human and macaque antibodies using antibody phage display for the detection of equine encephalitis viruses. Die Antikörperfabrik: Antikörper für jedes Protein. Viruses 10: 147, (125) Sadana, P., Geyer, R., Pezoldt, J., Helmsing, S., Huehn, J., Hust, M., Dersch, P. and Scrima, A. Aufbauend auf den Forschungsergebnissen des Konsortiums hat das Biotechnologieunternehmen YUMAB, eine Ausgründung der TU Braunschweig, die CORAT Therapeutics GmbH gegründet. An immunochemical in situ approach to detect adaptation processes in the photosynthetic apparatus of diatoms of the Wadden Sea sediment surface layers. Department of Biotechnology (134) Chauvin, D., Hust, M., Schütte, M., Chesnay, A., Parent, C., Moreira, G.M.S.G, Arroyo, J., Sanz, A.B., Pugnière, M., Martineau, P., Chandenier, J., Heuzé-Vourc’h, N. and Desoubeaux, G. (2019). and Hust, M.. (119) Russo, G., Meier, D., Helmsing, S., Wenzel, E., Oberle, F., Frenzel, A. and Hust, M. (2018). www.bbt.tu-bs.de/Biotech, Technische Universität Braunschweig A human scFv antibody generation pipeline for proteome research. (85) Miethe, S., Pelat, T., Avril, A., Popoff, M., Molgo, J., Rasetti‑Escargueil, C., Fontayne, A., Korkeala, H., Thullier, P., Sesardic, D. and Hust, M. (2014). Braunschweig. (2016). (128) Beer, L.A., Tatge, H., Schneider, C., Ruschig, M., Hust, M., Barton, J., Thiemann, S., Fühner, V., Russo, G. and Gerhard, R. (2018). (86) Froude, J.W., Pelat, T., Miethe, S., Zak, S., Bakken, R., Hust, M., Thullier, P. and Dye, J.M. “Finding such neutralizing antibody candidates was our most important milestone on the way to a passive immunotherapy against Covid-19, but there are still many more steps and tests to be done before the final drug is ready for use,” says Michael Hust. Affinity-matured variants derived from nimotuzumab keep the original fine specificity and exhibit superior biological activity. Gene Function Analysis, Ed: Ochs, M.F.. (76) Miethe, S., Rasetti-Escargueil, C., Liu, Y., Chahboun, S., Pelat, T., Avril, A., Frenzel, A., Schirrmann, T., Thullier, P., Sesardic, D. and Hust, M. (2014). (12) Konthur, Z., Hust, M. and Dübel, S. (2005). Antibodies produced by this method have the advantage that they are not synthetic or chemically produced substances, but are the body’s own molecules and therefore have hardly any side effects. However, since SARS-CoV-2 is a novel virus, many people have not yet developed antibodies against it. (69) Miethe, S., Meyer, T., Wöhl-Bruhn, S., Frenzel. Mining gut microbiome oligopeptides by functional metaproteome display. Mating antibody phage display with proteomics. (62) Frenzel, A., Fröde, D., Meyer, T., Schirrmann, T. and Hust, M. (2012). Dübel, S.. (23) Michalzik, M., Balck, A., Al-Halabi, L., Hust, M., Dübel, S. and Büttgenbach, S. (2007). Innovationspreis Niedersachsen. Fuctional knockdown of VCAM-1 at the posttranslational level with ER retained antibodies. The influence of antibody fragment format on phage display based affinity maturation of IgG.
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